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1.
Rev. bras. parasitol. vet ; 26(4): 511-515, Oct.-Dec. 2017. graf
Article in English | LILACS | ID: biblio-1042457

ABSTRACT

Abstract The trematodes are parasites of the several vertebrates including amphibians, however the knowledge about of the taxonomy these parasites is still confuse. The trematode Choledocystus elegans was found in the small intestine of the Leptodactylus paraensis in eastern Amazon and presents the following characteristics: several pointed tegumentary spines, papillae on the outer and inner edges of the oral and ventral suckers, a round, well-developed cirrus sac, a well-developed cirrus, oblique testicles, a ovary right side, uterine loops extending between the testicles, follicular vitellaria distributed throughout the body, starting at the genital pore region and caeca close the end of the body. For the first time, this study identified C. elegans parasitizing L. paraensis and describes morphological aspects never characterized using light and scanning electron microscopy.


Resumo Os trematodas são parasitas de vários grupos de vertebrados incluindo os anfíbios, contudo o que se conhece sobre a taxonomia destes parasitas ainda é confusa. O trematoda Choledocystus elegans foi encontrado no intestino delgado de Leptodactylus paraensis na Amazônia oriental e apresenta as seguintes características: vários espinhos pontiagudos no tegumento, papilas nas bordas externa e interna das ventosas orais e ventrais, bolsa do cirro bem desenvolvida contendo um cirro desenvolvido, testículos oblíquos, ovário destro, alças uterinas que se estendem entre os testículos, folículos vitelínicos distribuída por toda a lateral do corpo, começando ao nível do poro genital e cecos intestinais chegando até a região posterior do corpo do helminto. Pela primeira vez, este estudo identificou C. elegans parasitando L. paraensis e descreve aspectos morfológicos nunca caracterizados usando microscopia de luz comum e eletrônica de varredura.


Subject(s)
Animals , Trematoda/isolation & purification , Trematoda/anatomy & histology , Brazil , Anura/parasitology , Microscopy, Electron, Scanning
2.
Rev. bras. parasitol. vet ; 26(1): 74-80, Jan.-Mar. 2017. tab, graf
Article in English | LILACS | ID: biblio-844141

ABSTRACT

Abstract Leptodactylus syphax is distributed in central, southeastern and northeastern Brazil, eastern Bolivia and southern Paraguay, occupying open areas and rock outcrops, in rock cavities and termite burrows. We collected 21 frogs from the Caatinga region of the state of Ceará, northeastern Brazil, and 7,021 helminths were recovered from 18 of these hosts (overall prevalence = 85.7%). Six helminth taxa were recovered, as follows: Aplectana membranosa (n = 3,756); Schrankiana formosula (n = 3,176); larvae of Physaloptera sp. (n = 43); unidentified nematode larvae (n = 7); digenean metacercariae of Lophosicyadiplostomum sp. (n = 2); and cystacanths of Acanthocephala (n = 37). The similarity of helminth composition between L. syphax from the Caatinga and other species of the L. fuscus group showed that some anurans were clustered according to parasite species and others according to geographic locality. This study presents new helminth records for the Neotropical region, thus helping in understanding the pattern of species distribution, and it increases the knowledge of parasites associated with amphibians.


Resumo Leptodactylus syphax está distribuída na região central, Sudeste e Nordeste do Brasil, Leste da Bolívia e Sul do Paraguai, ocupando áreas abertas e afloramentos rochosos, cavidades rochosas ou de cupins. Foram coletadas 21 rãs oriundas da região de Caatinga do Ceará, nordeste brasileiro, e 7.021 helmintos foram recuperados em 18 hospedeiros (prevalência geral = 85,7%). Seis taxa de helmintos foram recuperados, como segue: Aplectana membranosa (n = 3.756), Schrankiana formosula (n = 3.176), larvas de Physaloptera sp. (n = 43), larvas de nematódeos não identificado (n = 7), metacercárias de Lophosicyadiplostomum sp. (n = 2), e cistacantos de Acantocéfalos (n = 37). A similaridade da composição de helmintos entre L. syphax da Caatinga e outras espécies do grupo L. fuscus mostrou que alguns anuros foram agrupados de acordo com a espécie do parasita e outros de acordo com a localidade geográfica. Este estudo apresenta novos registros de helmintos para a região Neotropical, ajudando na compreensão do padrão de distribuição das espécies e aumenta o conhecimento sobre os parasitas associados a anfíbios.


Subject(s)
Animals , Anura/parasitology , Ecosystem , Helminths/isolation & purification , Brazil , Acanthocephala/isolation & purification
4.
Braz. j. biol ; 75(1): 86-90, Jan-Mar/2015. tab, graf
Article in English | LILACS | ID: lil-744323

ABSTRACT

Amphibians are hosts for a wide variety of ecto- and endoparasites, such as protozoans and parasitic worms. Naididae is a family of Oligochaeta whose species live on a wide range of substrates, including mollusks, aquatic macrophytes, sponges, mosses, liverworts, and filamentous algae. However, some species are known as endoparasitic from vertebrates, such as Dero (Allodero) lutzi, which is parasitic of the urinary tracts of frogs, but also have a free-living stage. Specimens in the parasitic stage lack dorsal setae, branchial fossa, and gills. Here we report the occurrence of D. (A.) lutzi associated with anuran Scinax fuscovarius from Semi-deciduous Atlantic Rain Forest in southern Brazil. The study took place at the Caiuá Ecological Station, Diamante do Norte, Paraná, southern Brazil. Seven specimens of S. fuscovarius were examined for parasites but only one was infected. Parasites occurred in ureters and urinary bladder. Previous records of this D. (A.) lutzi include the Brazilian States of Santa Catarina, São Paulo, Rio de Janeiro, and Minas Gerais, as well as Cuba and North America. This is a new locality record for this species in Brazil. Reports of Dero (Allodero) lutzi are rare, due to difficulty of observation, and such events are restricted only the fortuitous cases. It is important to emphasize the necessity of future studies, which are fundamental to the understanding of biological and ecological aspects of this species.


Anfíbios são hospedeiros para uma grande variedade de ecto- e endoparasitos, tais como os protozoários e vermes parasitos. Naididae é uma família de Oligochaeta cujas espécies vivem em uma ampla variedade de substratos, incluindo moluscos, macrófitas aquáticas, esponjas, musgos, e algas hepáticas e filamentosas. No entanto, algumas espécies são conhecidas como endoparasitos de vertebrados, como Dero (Allodero) lutzi, o qual é um parasito do trato urinário de anfíbios, mas que também possui uma fase de vida livre. Espécimes no estágio parasitário não possuem cerdas dorsais, fossa branquial, e brânquias. Relatamos a ocorrência de D. (A.) lutzi associado ao anuro Scinax fuscovarius na Floresta Estacional Semidecidual no Sul do Brasil. O estudo foi realizado na Estação Ecológica do Caiuá, Diamante do Norte, Paraná, Sul do Brasil. Sete espécimes de S. fuscovarius foram examinados para parasitos, mas apenas um estava infectado. Os parasitos ocorreram nos ureteres e bexiga urinária. Registros anteriores de D. (A.) lutzi incluem os estados brasileiros de Santa Catarina, São Paulo, Rio de Janeiro e Minas Gerais, bem como Cuba e América do Norte. Este é o registro de uma nova localidade para esta espécie no Brasil. Registros de Dero (Allodero) lutzi são raros, devido à dificuldade de observação, e tais eventos são restritos apenas a casos fortuitos. É importante ressaltar a necessidade de futuros estudos, que são fundamentais para a compreensão de aspectos biológicos e ecológicos desta espécie.


Subject(s)
Animals , Anura/parasitology , Oligochaeta/classification , Anura/classification , Brazil
5.
An. acad. bras. ciênc ; 83(4): 1303-1312, Dec. 2011. ilus
Article in English | LILACS | ID: lil-607426

ABSTRACT

Proceratophrys boiei is an endemic cycloramphid anuran inhabiting the leaf litter of Atlantic rainforests in Southeastern Brazil. We analyzed the whole digestive tract of 38 individuals of Proceratophrys boiei collected in two Atlantic Rainforest areas in the state of Rio de Janeiro, Brazil, to study the diet composition and the helminth fauna associated with this species. The main food items in P. boiei's diet were Coleoptera, Orthoptera and Blattaria. Five nematode species were found: Aplectana delirae, Cosmocerca parva, Oxyascaris oxyascaris, Physaloptera sp. (larval stage only) and an unidentified nematode. Overall prevalence was 71 percent and mean infection intensity was 7.3 ± 5.8 neatodes per individual.


Proceratophrys boiei é um anuro da familia Cycloramphidae que vive no folhico e é endêmico de areas de floresta na Mata Atlantica do Sudeste do Brasil. Nós analisamos o trato digestivo de 38 indivíduos de Proceratophrys boiei provenientes de duas áreas de Mata Atlântica no Estado do Rio de Janeiro, Brasil, para estudar a composição da dieta e a fauna helmíntica associada a esta espécie. s principais itens alientares na dieta de P. boiei fora Coleoptera, rthoptera e Blattaria. Cinco espécies de nematóides foram encontradas: Aplectana delirae, Cosmocerca parva, Oxyascaris oxyascaris, Physaloptera sp. (apenas larvas) e uma espécie de nematóide não identificada. A prevalência total foi de 71 por cento e a intensidade media de infecção foi de 7,3 ± 5,8 nematóides por indivíduo.


Subject(s)
Animals , Anura/parasitology , Feeding Behavior/physiology , Nematoda/classification , Anura/anatomy & histology , Anura/classification , Anura/physiology , Brazil , Gastrointestinal Contents , Population Density , Trees
6.
Rev. biol. trop ; 59(1): 17-28, mar. 2011. ilus, tab
Article in Spanish | LILACS | ID: lil-638042

ABSTRACT

Hematology and blood cell cytochemistry of Rhinella fernandezae (Amphibia: Anura) from Espinal and Delta-Islands of Paraná River, Argentina. The description of amphibian hematology is scarce and most of these studies have been done in species from North America, Asia and Europe. With the purpose to obtain basic hematological information of Rhinella fernandezae, 23 blood samples from Santa Fe and Entre Ríos natural reserves were studied. Blood of each individual was extracted by cardiac puncture and hemograms were carried out. Morphological and cytochemical description of blood cells were analyzed in slides and were inspected for extra and intra cellular parasites. Five leucocytes types were observed, being lymphocytes the predominant ones followed by basophiles. Heterophils and eosinophils were positive to PAS, Sudan B and peroxidase. The erythrocytes and its precursors were negative for cytochemical reactions. Micronuclei and nuclear alterations frequencies were scarce. No significant differences (p>0.05) were observed between sexes neither in hemograms nor in blood cells morphology. Microfilarias were the only hemoparasites found with a relative low prevalence and infection intensity. The hematological characteristics studied were similar to those reported for other amphibians, suggesting that R. fernandezae individuals present optimal nutritional and immunological status. Rev. Biol. Trop. 59 (1): 17-28. Epub 2011 March 01.


La descripción de la hematología de los anfibios anuros es escasa, habiéndose realizado la mayoría de los trabajos en especies de Norteamérica, Asia y Europa. Con el propósito de obtener datos hematológicos para Rhinella fernandezae, fueron estudiados 23 especímenes provenientes de zonas protegidas de las provincias de Santa Fe y Entre Ríos. Se les extrajo sangre por punción cardíaca y se realizaron hemogramas. En los extendidos sanguíneos, se efectuaron la descripción morfológica y citoquímica de las células sanguíneas y búsqueda de parásitos. Se observaron cinco tipos de leucocitos, donde predominaron los linfocitos pequeños. Heterófilos y eosinófilos resultaron positivos para PAS, Sudan B y peroxidasa; contrariamente, los eritrocitos y sus precursores fueron negativos. Los puntajes de las reacciones citoquímicas fueron variables para basófilos, linfocitos, monocitos y trombocitos. Las frecuencias de micronúcleos y de alteraciones nucleares fueron escasas. No se observaron diferencias significativas (p>0.05) entre sexos en el hemograma ni en la morfología sanguínea. Los únicos hemoparásitos encontrados fueron microfilarias (Nematoda: Filaroidea), cuya prevalencia e intensidad de infección fueron bajas. Las características hematológicas estudiadas fueron semejantes a los valores reportados para otros anfibios, pudiendo inferir que los individuos de R. fernandezae estudiados se encuentran en un buen estado nutricional e inmunológico.


Subject(s)
Animals , Female , Male , Anura/blood , Blood Cells , Hematologic Tests/veterinary , Histocytochemistry/veterinary , Argentina , Anura/parasitology , Blood Cells/chemistry , Blood Cells/cytology , Blood Cells/parasitology , Reference Values , Rivers
7.
An. acad. bras. ciênc ; 81(2): 199-206, June 2009. ilus, tab
Article in English | LILACS | ID: lil-514654

ABSTRACT

Wild animals are exposed to numerous pathogens, including hemoparasites. The Trypanosoma and hemogregarinegroup are frequently reported as parasites in anurans (frogs, tree frogs and toads). The identification of these hemoparasites is usually made through stage observation of their morphology in the peripheral blood of the host. There areno studies, however, based on the biological cycle of these hemoparasites. The objective of the present study was toevaluate the presence of hemogregarines and Trypanosoma spp. in anurans captured in the States of São Paulo andMato Grosso do Sul- Brazil and to perform the morphological and morphometric characterization of these hemoparasites. The species of anurans examined were: Dendropsophus nanus, D. minutus, Leptodactylus chaquensis L. podicipinus, L. labyrinthicus, L. fuscus, Bufo granulosus, B. schneideri, Phyllomedusa hypocondrialis, Trachicephalus venulosus, Scinax fuscovarius and Hypsiboas albopunctatus. Of the total of 40 animals studied, four (10 percent)were positive for hemogregarines and eight (20 percent) were positive for Trypanosoma spp. Hemogregarine gamontsshowed variable morphology and, in addition to intraerythrocytic forms, extraerythrocytic forms were also observed.Extremely different forms of Trypanosoma were observed, as described in the literature, with the broad and oval forms being the most common.


Os animais silvestres estão expostos a inúmeros patógenos,dentre eles estão os hemoparasitas. Podem-se destacar espécies do gênero Trypanosoma e do grupo das hemogregarinas,que ocorrem com freqüência parasitando anuros (rãs, pererecas e sapos). Normalmente, a descrição destes hemoparasitas é feita através da morfologia dos estágios observados nosangue periférico do hospedeiro e as pesquisas sobre o ciclobiológico desses hemoparasitas são escassas. Os objetivos dopresente estudo foram avaliar a presença de hemogregarinas eTrypanosoma spp. em anuros capturados nos Estados de São Paulo e Mato Grosso do Sul e fazer a caracterização morfológica e morfométrica dos seus hemoparasitas. As espécies deanuros examinadas foram: Dendropsophus nanus, D. minutus, Leptodactylus chaquensis, L. podicipinus, L. labyrinthicus, L. fuscus, Bufo granulosus, B. schneideri, Phyllomedusahypocondrialis, Trachicephalus venulosus, Scinax fuscovarius e Hypsiboas albopunctatus. Dos 40 animais estudados, foramencontrados quatro (10 por cento) positivos para hemogregarinas e oito(20 por cento) positivos para Trypanosoma spp. Foram observadosgamontes de hemogregarinas com morfologia variável e, alémdas formas intraeritrocíticas, também foram observados gamontes fora das hemácias. As formas de Trypanosoma encontradas eram muito polimórficas, conforme é descrito na literatura, sendo na sua maioria, larga e oval.


Subject(s)
Animals , Anura/parasitology , Coccidia/isolation & purification , Trypanosoma/isolation & purification , Anura/classification , Brazil
8.
Rev. biol. trop ; 56(4): 2147-2161, Dec. 2008. ilus, tab
Article in English | LILACS | ID: lil-637803

ABSTRACT

The nematological fauna of most anuran species from Corrientes province, north of Argentina; has not been studied. We report for the first time the nematode species found in Rhinella schneideri and Scinax acuminatus. Forty four amphibians representing two species (R. schneideri -six males, three females and two juveniles- and S. acuminatus -fifteen males and eighteen females) were collected near the city of Corrientes, between January 2002 and December 2003 and searched for nematodes. R. schneideri contained eight species of nematodes (adults: Rhabdias füelleborni, R. elegans, Oswaldocruzia proencai, Cosmocerca podicipinus, C. parva and Falcaustra mascula; larvae: Porrocaecum sp. and Physaloptera sp.), and S. acuminatus contained three (adults: Cosmocerca parva and Oxyascaris caudacutus; larvae: Physaloptera sp.). We present morphology (scanning electron microscope) and metric information, range extensions, and new host records for these nematode species. Rev. Biol. Trop. 56 (4): 2147-2161. Epub 2008 December 12.


Cuarenta y cuatro anfibios pertenecientes a dos especies (Rhinella schneideri -seis machos, tres hembras y dos juveniles- y Scinax acuminatus -quince machos y dieciocho hembras) fueron recolectados para extraer nemátodos en las proximidades de la ciudad de Corrientes, provincia de Corrientes en Argentina, entre enero 2002 y diciembre 2003. Rhinella schneideri estuvo parasitada por ocho especies de nemátodos (adultos: Rhabdias füelleborni, R. elegans, Oswaldocruzia proencai, Cosmocerca podicipinus, C. parva y Falcaustra mascula; larvas: Porrocaecum sp. y Physaloptera sp.), y S. acuminatus presentó tres especies de nemátodos (adultos: Cosmocerca parva y Oxyascaris caudacutus; larva: Physaloptera sp.). Para todas estas especies de nemátodos se presentan datos morfológicos y métricos, y para algunas sus nuevos ámbitos y caracteres, así como también los detalles obtenidos mediante el microscopio electrónico de barrido. Éste es el primer informe de nemátodos parásitos para los citados anfibios de Corrientes, Argentina.


Subject(s)
Animals , Female , Male , Anura/parasitology , Nematoda/isolation & purification , Argentina , Nematoda/anatomy & histology , Nematoda/classification
10.
Braz. j. biol ; 66(1a): 85-93, Feb. 2006. ilus, tab, graf
Article in English | LILACS | ID: lil-426269

ABSTRACT

De dezembro de 1995 a novembro de 2000, a maturação sazonal de Glypthelmins vitellinophilum Dobbin, 1958, em seu hospedeiro definitivo, a rã Lysapsus limellus Cope, 1862, foi estudada em uma lagoa permanente subtropical no nordeste da Argentina. Os objetivos deste estudo foram: 1) determinar a dinâmica de infrapopulação do parasita, analisando o ciclo de maturação sazonal ao longo dos anos; e 2) examinar a relação entre intensidade de infecção do trematódeo em diferentes estádios do desenvolvimento (recrutamento, crescimento e maturação) e o comprimento de corpo da rã. De 1.400 espécimens de rãs em 60 meses (5 anos), foram achados 38% infetados com G. vitellinophilum, e a intensidade de infecção era 1-15 trematódeos por rã. Espécimes de G. vitellinophilum estavam presentes em L. limellus ao longo dos anos, mas não mostraram um ciclo sazonal pronunciado de maturação. São discutidas possíveis razões para estes resultados com referência a flutuações climáticas e fatores bióticos. O período infectante do parasita (estádio I) aconteceu no verão, outono e primavera, coincidentemente com o tempo de aparecimento de cada corte das rãs. Estas infecções eram principalmente registradas em tamanhos de corpo pequenos (classes 1 e 2) de L. limellus. Espécimes de parasitas juvenis e não grávidas (estádios II e III) eram encontradas em diferentes tamanhos de rãs ao longo do período de investigação. Espécimes de parasitas grávidas (estádio IV) eram geralmente registradas no outono, inverno e primavera, principalmente nos tamanhos de corpo maiores de rãs. O comprimento de corpo do trematódeo dos estádios I e IV eram significativa e positivamente correlatos com o comprimento de corpo da rã.


Subject(s)
Pregnancy , Animals , Female , Anura/parasitology , Trematoda/growth & development , Trematode Infections/veterinary , Argentina , Host-Parasite Interactions , Seasons , Trematoda/physiology , Trematode Infections/parasitology
11.
Braz. j. biol ; 66(1a): 167-173, Feb. 2006. tab, graf
Article in English | LILACS | ID: lil-426279

ABSTRACT

Analisamos a composição da dieta, endoparasitas e dimorfismo sexual em tamanho no anuro Chiasmocleis capixaba (Microhylidae) em um habitat de "mussununga" no município de Nova Viçosa, no sul do Estado da Bahia, Brasil. Todos os 119 espécimes analisados foram coletados em uma única noite com chuva intensa. As fêmeas (comprimento rostro-cloacal médio = 15,7 + 3,0 mm) foram significativamente maiores que os machos (comprimento rostro-anal médio = 13,2 + 2,1 mm), e indivíduos de ambos sexos foram menores do que os de uma população coespecífica de Aracruz, no Estado do Espírito Santo. A dieta de C. capixaba estava dominada por ácaros, formigas e colêmbolos. Setenta e nove indivíduos (66.4% do total) estavam infectados por helmintos, todos pertencentes a uma única espécie, Cosmocerca ornata, um nematódeo intestinal.


Subject(s)
Animals , Male , Female , Anura , Body Size , Feeding Behavior , Sex Characteristics , Anura/anatomy & histology , Anura/parasitology , Anura/physiology , Brazil , Gastrointestinal Contents , Trees
12.
The Korean Journal of Parasitology ; : 127-131, 2006.
Article in English | WPRIM | ID: wpr-113936

ABSTRACT

The furcocercus cercariae of Neodiplostomum seoulense (Digenea: Neodiplostomidae) penetrate the skins of tadpoles and shed their tails. The speculated mechanism of this tail loss was physical efforts required to produce a vigorous zigzag motion during skin penetration; no other mechanism has been proposed. We examined the relationship between the host serum and cercarial tail loss. Cercariae of N. seoulense were collected from experimentally infected Segmentina hemisphaerula, and lots of 300 cercariae were cultured in medium 199 contained several types of sera. Cercarial tail degradation was induced in all media, but all the cercariae cultured except those cultured in media containing fetal bovine serum (FBS) died within 48 hr. After 72 hr cultivation in media containing FBS, cercarial tail degradation was induced in 67.0%; in continuous cultivation 13.3% of larvae survived for 7 days. Tail degradation did not occur in the absence of serum and when serum was heat inactivated at 56 degrees C for 30 min. The addition of 20 mM ethylenediaminetetraacetic acid (EDTA) blocked cercarial tail degradation completely. Moreover, the addition of 20 mM MgCl2 restored tail degradation blocked by EDTA. These results suggest that the alternative complement pathway is related with the N. seoulense cercarial tail degradation induced by serum.


Subject(s)
Animals , Trematoda/physiology , Tail/physiology , Larva/parasitology , Complement System Proteins/immunology , Anura/parasitology
13.
Iranian Journal of Veterinary Research. 2005; 6 (3): 67-73
in English | IMEMR | ID: emr-71214

ABSTRACT

A total of 108 toads and frogs were collected by hand from January to August 1994 from different sites in the southwest of Iran, mainly from Khouzestan province. Among 79 green toads [Bufo viridis], 93.7% were infected with one or more parasite species. There was no significant difference between snout-ventral lengths [SVL] of infected [n = 74] and uninfected [n = 5] toads [P = 0.323; Student's t-test], also between the rate of infection in males and females [P = 0.392; chi 2 test]. Helminth parasites of B. viridis were the monogenean, Polystoma viridis, the digenean, Haplometra cylindracea the cyclophyllid cestodes, Nematotaenia dispar; Diplopylidium acanthotetra cysticercoid and the nematodes, Cosmocerca ornata, Cosmocerca commutata, Rhabdias bufonis and Aplectana sp. There was no significant difference between the rate of infection with P. viridis [chi 2 = 1.92, P = 0.166] and N. dispar [chi 2 = 0.032, P = 0.859] in males and females. Males [n = 12], however, were significantly more infected [chi 2 = 5.52, P = 0.019] with R. bufonis than females [n = 8]. SVL of B. viridis infected with N. dispar and uninfected samples were significantly different [P = 0.016]. No significant difference was shown for infection with P. viridis [P>0.05; Student's t-test]. Among 17 marsh frogs [Rana ridibunda ridibunda], 58.8% were infected with helminth parasites. There was no significant difference between SVL of infected [n = 10] and uninfected [n = 7] frogs [P = 0.238; Student's t-test] and also between uninfected males and females using [P = 0.201; chi 2 test]. Metacercarian cysts of digenetic trematode Codonocephalus urniger and the nematode Aplectana sp. were found in Rana ridibunda ridibunda specimens. Twelve true tree frogs, Hiyla arborea savignyi were examined. The monogenean, Polystoma viridis, the cyclophyllid cestode, Nematotaenia dispar and the nematode, Aplectana sp. were found in the tree frogs


Subject(s)
Animals , Helminths , Bufonidae/parasitology , Ranidae/parasitology , Anura/classification , Anura/parasitology
15.
Mem. Inst. Oswaldo Cruz ; 93(3): 357-61, May-Jun. 1998. ilus
Article in English | LILACS | ID: lil-209957

ABSTRACT

Intestine samples of Bufo sp. tadpoles with parasitism confirmed for Giardia agilis were studied by transmission electron microscopy. The G. agilis trophozoites were long and thin. The plasma membrane was sometimes undulated and the cytoplasm, adjacent to the dorsal and ventral regions, showed numerous vacuoles. The two nuclei presented prominent nucleoli. The cytoplasm was electron-dense with free ribosomes, glycogen and rough endoplasmic reticulum-like structures. Polyhedral inclusions were observed in the cytoplasm and outside the protozoan; some of these inclusions exhibited membrane disruption. The flagella ultrastructure is typical, with the caudal pair accompanied by the funis. Next to the anterior pair, osmiophilic material was noticed. The ventro-lateral flange was short and thick, supported by the marginal plates that penetrated into its distal extermity; only its distal portion had adjacent osmiophilic filament. The G. agilis trophozoites showed the general subcellular feature of the genus. However, the ventro-lateral flange ultrastructure was an intermediate type between G. muris and G. duodenalis.


Subject(s)
Animals , Amoeba/ultrastructure , Giardia/ultrastructure , Intestines/parasitology , Anura/parasitology
16.
Mem. Inst. Oswaldo Cruz ; 92(6): 767-70, Nov.-Dec. 1997. ilus
Article in English | LILACS | ID: lil-197213

ABSTRACT

A new species found in the intestine of Eupsophus calcaratus (Anura: Leptodactylidae) from Yaldad, Chile (45º5'S;73º43'W) is described. This nematode is compared with other South American species of the genus. Aplectana artigasi differs from the only known Chilean species, Aplectana chilensis, as the former has a greater number of postanal papillae and double papilla in the ventral region of the tail.


Subject(s)
Animals , Nematoda/ultrastructure , Anura/parasitology
18.
Bol. chil. parasitol ; 51(1/2): 34-5, ene.-jun. 1996.
Article in Spanish | LILACS | ID: lil-185096

ABSTRACT

The chilean leptodactylid frog eupsophus calcaratus, a typical inhabitant of the temperate forest of South America, is registered as a new host of baerietta chilensis (cestoda: nematotaeniidae)


Subject(s)
Animals , Anura/parasitology , Cestoda/isolation & purification , Cestoda/classification , Host-Parasite Interactions
19.
Parasitol. día ; 20(1/2): 59-62, ene.-jun. 1996. tab
Article in Spanish | LILACS | ID: lil-185267

ABSTRACT

Pleurodema thaul Lesson, 1826 es una especie de amplia distribución geográfica y gran variabilidad morfológica, por lo cual, se plantea el estudio de su parasitofauna para contribuir a dilucidar el estado taxonómico de las diferentes poblaciones. En este trabajo se da a conocer la composición taxonómica de la parasitofauna encontrada en una población local de P. thaul, ubicada en Concepción, además de características cuantitativas (prevalencia, intensidad y abundancia) y microhabitat de los taxa parasitarios encontrados. Se encontraron tres especies de nemátodos: aplectan chilensis, rhabdias sp. y oswaldocruzia sp. más una especie de protozoo coccidio del género eimeria, constituyendo los primeros registros para P thaul


Subject(s)
Animals , Male , Female , Anura/parasitology , Eukaryota/isolation & purification , Nematoda/isolation & purification , Intestinal Mucosa/parasitology , Prevalence , Host-Parasite Interactions , Gallbladder/parasitology
20.
Mem. Inst. Oswaldo Cruz ; 90(5): 653-5, set.-oct. 1995. ilus
Article in English | LILACS | ID: lil-157287

ABSTRACT

Blood erythrocytes of Brazilian tree-frogs, Phrynohyas venulosa were found to frequently contain single, small, densely staining inclusions. Electron microscopy showed these to be icosahedral viral particles which measured from 250-280 nm in diameter; they were devoid of an envelope, and thus differed from previously described viruses of frog erythrocytes. The infected erythrocytes lacked a crystalline body.


Subject(s)
Animals , Anura/parasitology , Erythrocytes/physiology , Microscopy, Electron
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